Oct 24, 2019 AAV-TRE-fDIO-GFP-IRES-tTA (109 gc/ml) virus were mixed with the ratio of 1:9 . 200 nL mixed virus was injected into the DR for each mouse.

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1 Jun 2019 linear gradient consisting of 1–100% solvent B over 180 min at a flow rate of 200 nL/min. Full MS scans were BMA:6 NAG:48. BMA:6 NAG:54.

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acquired using BMA-931 biopotential amplifier (Dataq. Instruments, Akron, OH 200 nL ( $1000 viral particles) were injected into the left hemisphere over a 

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Full MS scans were BMA:6 NAG:48.
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methacrylate (BMA) were purchased from Alfa Aesar (Ward Hill, MA). To reduce the gradient delay time at 200 nL/min, we actually utilized the gradient  Oct 29, 2019 BMA basomedial amygdalar nucleus, CA1/CA3 CA1/CA3 area of the was calibrated and set to 5 µl/min; injection volume was 200 nl/site.